“Bioline,siRNA转染试剂,假病毒包装,荧光定量PCR试剂,唾液DNA提取试剂”/

“Bioline,siRNA转染试剂,假病毒包装,荧光定量PCR试剂,唾液DNA提取试剂”/
核酸转染
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RFect siRNA/miRNA Transfection Reagent

RFect小核酸转染试剂

货号规格(ml)目录价(元)网购价(元)
110110.51,080810
1101211,8301,373
110131.52,5901,943

产品简介

RFect sRNA Transfection Reagent是国际知名科学家崔坤元博士领导我公司研发团队在美国西雅图实验室研发成功的一种新型的小核酸转染试剂。RFect可用来转染siRNA、antisenseRNA、microRNA等200bp以内的小分子RNA和DNA,转染细胞包括绝大多数贴壁生长的细胞,如一般细胞株、肿瘤细胞株等。目前,无论国外还是国内,转染试剂的主要成分均为脂质体或聚乙烯亚胺(Polyethylenimine, PEI),这两种成分都具有很大的细胞毒性,并且转染效果不好。RFect采用新型的动物源性的纳米材料,毒性很低并且拥有非常卓越的转染性能。与其它品牌的小核酸转染试剂相比,RFect的细胞转染阳性率一般在90%以上,Lamin A/C基因抑制效率在95%以上,而其它品牌转染试剂的细胞转染阳性率一般不超过70%,Lamin A/C 基因抑制效率不超过75%。RFect细胞毒性很低,转染细胞死亡率不到10%,而其它品牌试剂的转染细胞死亡率一般在30%以上。另外,RFect使用极其简便,先将siRNA与RFect室温混合,再将siRNA-RFect混合物直接加入含培养基的细胞,血清对转染效果没有影响,不必刻意添加或更换培养液。有关RFect sRNA Transfection Reagent的材料合成和试剂配制我们已申请了国际专利,并通过PCT覆盖国际上多个国家和地区。

适用细胞株

293T(人肾上皮细胞)A549(人肺癌细胞)HEK 293(人胚肾细胞),HeLa(人宫颈癌细胞)MCF-7(人乳腺癌细胞)HepG2(人肝肿瘤细胞)HT-1080(人纤维肉瘤)HLF-a(人肺细胞)Huh-7(人肝癌细胞)KB(人口腔表皮样癌细胞)PC3(人前列腺癌细胞)BRL-3A(大鼠正常肝细胞),DU 145(人前列腺癌细胞)Hepa 1-6(小鼠肝癌细胞)OVCAR3(人卵巢癌细胞)NCaP-FGC(人前列腺癌细胞),C2C12(成肌细胞),MDCK(犬肾细胞),CHO-K1islk.219细胞(人肺癌细胞),HT-29(人结肠癌细胞),NCI-H1688(人典型小细胞肺肿瘤/癌细胞),RKO结肠癌细胞,Vero(非洲绿猴肾细胞),COS-1(非洲绿猴肾成纤维细胞),COS7-L(非洲绿猴肾成纤维细胞),COS-7(非洲绿猴肾成纤维细胞),SK-BR3(人乳腺癌细胞),Hepa 1cLc7(鼠肝癌细胞),Daoy(人髓母细胞瘤细胞),DBTRG-05MG(人脑胶质母细胞瘤细胞),KLN 205(鼠肺磷癌细胞),Neuro-2a(小鼠神经瘤母细胞)DI-TNC1(大鼠脑间质细胞)

产品特点

1. 卓越的细胞转染性能:细胞转染阳性率可高达90%以上,基因敲除效果明显,A549细胞Lamin A/C基因敲除效率在95%以上;

2. 极低的细胞毒性使实验结果更为客观:转染细胞死亡率不到10%,大大降低了因细胞毒性对实验结果的影响。

3. 转染细胞范围非常广,绝大多数贴壁细胞株都能获得比较理想的转染结果。

 

 

 

 

 

 

 

 

 

Fig. 1. RFect sRNA transfection reagent offers the highest transfection efficiency.  HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using RFect sRNA transfection reagent. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A The fluorescence images show the fluorescence of labeled siRNA.B.The fluorescence images show the fluorescence of labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).

 

 

 

 

 

 

 

 

Fig.2. RFect sRNA transfection reagent offers high level of gene knockdown with low concentration of siRNA.Lamin A/C siRNA-RFect transfection complexes were prepared in 96-well plates, and then A549 cells were added to each well.

 

 

 

 

 

 

 

 

 

Fig.3. RFect sRNA transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFect  In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

相关文献

1.Cell Research.(2017)27.5-methylcytosine promotes mRNA export-NSUN2 as the methyltransferase and ALYREF as an m5C reader.(IF:14.812)

2.Nucleic Acids Research.2015 May 12.XPD localizes in mitochondria and protects the mitochondrial genome from oxidative DNA damage.(IF:9.202)

3.Scientific Reports.2013 Jan 1.APG:an Active Protein-Gene Network Model to Quantify Regulatory Signals in Complex Biological Systems.(IF:5.078)

4.Oncotarget.2016.Knockdown of golgi phosphoprotein 2 inhibits hepatocellular carcinoma cell proliferation and motility.(IF:5.008)

相关产品链接

RFectPM原代细胞小核酸转染试剂 (针对原代细胞)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=20

RFect质粒DNA转染试剂盒(新产品 针对普通肿瘤细胞株、原代细胞、悬浮细胞质粒DNA转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=167

DeofectEU真核细胞DNA转染试剂 (针对大多数真核细胞质粒DNA转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=23

RFectSP悬浮细胞小核酸转染试剂(针对悬浮细胞)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=158

肝素钠Heparin sodium salt (心脑血管研究)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=148

 

 

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