RFect siRNA/miRNA Transfection Reagent

RFect小核酸转染试剂

产品简介

RFect小核酸转染试剂是国际知名科学家崔坤元博士领导我公司研发团队在美国西雅图实验室研发成功的一种新型的小核酸转染试剂。RFect可用来转染siRNA、miRNA mimics、miRNA inhibitor 等200bp以内的小分子RNA，转染细胞包括绝大多数贴壁生长的细胞，如一般细胞株、肿瘤细胞株等。目前，无论国外还是国内，转染试剂的主要成分均为脂质体或聚乙烯亚胺(Polyethylenimine, PEI)，这两种成分都具有很大的细胞毒性，并且转染效果不好。RFect采用新型的动物源性的纳米材料，毒性很低并且拥有非常卓越的转染性能。与其它品牌的小核酸转染试剂相比，RFect的细胞转染阳性率一般在90%以上，Lamin A/C 基因抑制效率在95%以上，而其它品牌转染试剂的细胞转染阳性率一般不超过70%，Lamin A/C 基因抑制效率不超过75%。RFect细胞毒性很低，转染细胞死亡率不到10%，而其它品牌试剂的转染细胞死亡率一般在30%以上。血清对转染效果没有影响，不必刻意添加或更换培养液。本产品适用于细胞株转染，原代细胞转染，请选择RFectPM小核酸转染试剂。

产品特点

· 卓越的细胞转染性能：细胞转染阳性率高达90%以上，基因敲除效果明显，A549细胞Lamin A/C 基因敲除效率在95%以上；

· 极低的细胞毒性：转染细胞死亡率不到10%，大大降低了因细胞毒性对实验结果的影响；

· 转染细胞范围广，绝大多数贴壁细胞株都能获得比较理想的转染结果。

Fig. 1. RFect sRNA transfection reagent offers the highest transfection efficiency.  HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using RFect sRNA transfection reagent. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A The fluorescence images show the fluorescence of labeled siRNA.B.The fluorescence images show the fluorescence of labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).

Fig..2. RFect siRNA transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFect  In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

 Fig..3.RFect siRNA transfection reagent offers high level of gene knockdown with low toxicity. A549 cell lines were transfected with Lamin A/C siRNA (final concentration: 10nM) using RFect siRNA transfection reagent. RFect concentrations added to the cells  exceeding transfection concentration of 20 times, the cells still do not produce significant toxicity, which makes these product suitable to optimization. Lamin A/C knockdown was measured by qRT-PCR.

Fig.4.RFect siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using RFect.

适用细胞

293T（人肾上皮细胞），A549（人肺癌细胞），HEK 293（人胚肾细胞），HeLa（人宫颈癌细胞），MCF-7（人乳腺癌细胞），HepG2（人肝肿瘤细胞），HT-1080(人纤维肉瘤），HLF-a（人肺细胞），Huh-7（人肝癌细胞），KB（人口腔表皮样癌细胞），PC3（人前列腺癌细胞），BRL-3A（大鼠正常肝细胞），DU 145（人前列腺癌细胞），Hepa 1-6（小鼠肝癌细胞），OVCAR3（人卵巢癌细胞），NCaP-FGC（人前列腺癌细胞），C2C12（成肌细胞），MDCK（犬肾细胞），CHO-K1，islk.219细胞（人肺癌细胞），HT-29（人结肠癌细胞），NCI-H1688（人典型小细胞肺肿瘤/癌细胞），RKO结肠癌细胞，Vero（非洲绿猴肾细胞），COS-1（非洲绿猴肾成纤维细胞），COS7-L（非洲绿猴肾成纤维细胞），COS-7（非洲绿猴肾成纤维细胞），SK-BR3（人乳腺癌细胞），Hepa 1cLc7（鼠肝癌细胞），Daoy（人髓母细胞瘤细胞），DBTRG-05MG（人脑胶质母细胞瘤细胞），KLN 205（鼠肺磷癌细胞），Neuro-2a（小鼠神经瘤母细胞），DI-TNC1（大鼠脑间质细胞）等。

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相关产品链接

RFect^PM小核酸转染试剂（国际专利技术，针对原代细胞siRNA、antisenseRNA、microRNA等转染）

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=20

RFect^SP小核酸转染试剂（国际专利技术，针对悬浮细胞siRNA、antisenseRNA、microRNA等转染）

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=158

RFect质粒DNA转染试剂盒（新产品！国际专利技术， 针对培养细胞质粒DNA转染）

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=167

RFect^PM原代细胞DNA转染试剂盒(新产品！国际专利技术， 针对原代细胞质粒DNA转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=189