“Bioline,siRNA转染试剂,假病毒包装,荧光定量PCR试剂,唾液DNA提取试剂”/

“Bioline,siRNA转染试剂,假病毒包装,荧光定量PCR试剂,唾液DNA提取试剂”/
核酸转染
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RFectPMsiRNA/miRNA Transfection Reagent

RFectPM原代细胞小核酸转染试剂

货号规格(ml)目录价(元)

 网购价(元)

(电议)

110140.51,580电议
1101512,820电议
110161.54,020电议

产品简介

RFectPM原代细胞siRNA转染试剂是在RFect siRNA转染试剂的基础上加以改进研发成功的专门用于原代细胞小核酸转染的试剂。RFectPM可用来转染siRNA、antisense RNA、microRNA等200bp以内的小分子RNA和DNA。RFectPM使用极其简便,先将siRNA与RFectPM室温混合,再将siRNA-RFectPM混合物直接加入含培养基的细胞,血清对转染效果没有影响,不必刻意添加或更换培养液。有关RFectPM原代细胞siRNA转染试剂的材料合成和试剂配制我们已申请了专利,并通过PCT覆盖多个国家和地区。 

产品特点

· 很好的原代细胞转染性能:原代细胞转染阳性率一般在80%以上,基因敲除效果明显,肝细胞LaminA/C基因敲除效率在90%以上;

· 极低的细胞毒性使实验结果更为客观:转染细胞死亡率不到10%;

· 低浓度siRNA转染获得高基因抑制水平;

· 转染细胞范围广泛,绝大多数原代细胞都能获得比较理想的转染结果。对一些活体寄生虫幼虫,RFectPM也能获得较为理想的转染结果。

Fig.1. Superior knockdown with RFectPM transfection reagent on difficult transfection primary cells.Differentprimarycells were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFectPM.In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

Fig.2. RFectPM transfection reagent offers high level of gene knockdown with low concentration of siRNA. Lamin A/C siRNA-RFectPM transfection complexes were prepared in 96-well plates,and then bovine chondrocytes were added to each well.

适用细胞

Clone 9(大鼠肝细胞),HUVEC(人脐静脉原代内皮细胞),原代血管平滑肌细胞,大鼠原代心肌细胞,Primary Human Fibroblasts(人原代成纤维细胞),大鼠骨髓间充质干细胞,MSC(间充质干细胞),BE(2)C(人神经母细胞瘤细胞),MRC-5(人胚胎成纤维细胞),NIH3T3(小鼠胚胎成纤维细胞),B16F10(小鼠黑色素瘤高转移细胞),BNL.CL2(小鼠胚胎肝细胞),C6(大鼠神经胶质瘤细胞),神经元细胞等。

相关文献

1.International Journal of Biological Sciences.(2021)KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer( IF:4.858 ) DOI: 10.7150/ijbs.54074

2.Frontiers in Cell and Developmental Biology.(2020)CRLF1 Is a Key Regulator in the Ligamentum Flavum Hypertrophy.(IF:5.201)DOI: 10.3389/fcell.2020.00858

3.Fish & Shellfish Immunology Pub Date(2020)Chlorpyrifos induces apoptosis and autophagy in common carp lymphocytes by influencing the TCR γ-dependent PI3K/AKT/JNK pathway.DOI: 10.1016/j.fsi.2020.02.039

4.Molecular Medicine Reports.(2019)BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NFκB signaling pathway in glomerular mesangial cells.(IF:2.1)DOI: 10.3892/mmr.2019.10870

5.Oxidative Medicine and Cellular Longevity.(2019)Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite.(IF:5.076)DOI: 10.1155/2019/5769752

6.Journal of Experimental & Clinical Cancer Research.(2018)INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia.(IF:7.068)DOI: 7.1186/s13046-018-0675-9

7.Cell Research.(2017)27.5-methylcytosine promotes mRNA export-NSUN2 as the methyltransferase and ALYREF as an m5C reader.(IF:14.812)

8.Journal of Molecular Endocrinology.(2017)SP600125 suppresses Keap1 e­xpression and results in NRF2-mediated prevention of diabetic nephropathy.(IF:3.562)DOI: 9.1530/jme-17-0260

9.Oncotarget.2016.Knockdown of golgi phosphoprotein 2 inhibits hepatocellular carcinoma cell proliferation and motility.(IF:5.008)

10.Nucleic Acids Research.2015 May 12.XPD localizes in mitochondria and protects the mitochondrial genome from oxidative DNA damage.(IF:9.202)

11.Scientific Reports.2013 Jan 1.APG:an Active Protein-Gene Network Model to Quantify Regulatory Signals in Complex Biological Systems.(IF:5.078)



相关产品链接

RFect细胞株小核酸转染试剂(国际专利技术,针对贴壁细胞siRNA、antisenseRNA、microRNA等转染

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=19

RFectSP小核酸转染试剂(国际专利技术,针对悬浮细胞siRNA、antisenseRNA、microRNA等转染

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=158

RFect质粒DNA转染试剂盒(新产品!国际专利技术, 针对培养细胞质粒DNA转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=167

RFectPM原代细胞质粒DNA转染试剂盒(新产品!国际专利技术, 针对原代细胞质粒DNA转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=189


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